Figure 4

Histological analyses of ovary, liver, and stomach from WT, control, and experimental female mice. (A) Ovarian histology of an 8 wk old WT mouse. The ovary contains follicles at various developmental stages. Arrows indicate granulosa cells and arrowheads denote oocytes surrounded by a magenta-colored zona pellucida. (B and C) Ovarian histology of a 26 wk old Smad2^flox/-; Inha^-/-mouse (B) and a 23 wk old Smad2^flox/-; Inha^-/-; Amhr2^cre/+mouse (C), respectively. Tumors from both genotypes were bilateral, large, hemorrhagic, and histologically indistinguishable. (D-F) Histology of livers from a 12 wk old WT mouse (D), a 15 wk old Smad2^flox/-; Inha^-/-mouse (E), and an 8 wk old Smad2^flox/-; Inha^-/-; Amhr2^cre/+mouse (F). Hepatocellular death around the central vein and lymphocyte infiltration are present in the livers of both the Smad2^flox/-; Inha^-/-control and Smad2^flox/-; Inha^-/-; Amhr2^cre/+mice (arrows). (G-I) Glandular stomachs from a 12 wk old WT mouse (G), a 26 wk old Smad2^flox/-; Inha^-/-mouse (H), and a 14 wk old Smad2^flox/-; Inha^-/-; Amhr2^cre/+mouse (I). Note the depletion of parietal cells in both the control and experimental mice (single asterisk) in comparison with the WT mouse (the large and eosinophilic cells; double asterisks). The junction ridge between the squamous epithelium of the forestomach and the glandular stomach is indicated by blue arrows. Scale bars = 100 μm.