Figure 5From: Downregulation of both gene expression and activity of Hsp27 improved maturation of mouse oocyte in vitroImmunofluorescence staining of cleaved-caspase 3 in oocytes after microinjecting of Hsp27 antibody (The other factors not shown). Oocytes were fixed in 4% paraformaldehyde after microinjecting of Hsp27 antibody for 36-48 h. Then these oocytes were stained with cleaved-caspase 3 antibody (green). Chromosome material was counterstained with propidium iodide (red). A) IgG-injected oocyte. B) Hsp27 antibody-injected oocyte.Back to article page