Figure 2From: Downregulation of both gene expression and activity of Hsp27 improved maturation of mouse oocyte in vitroLocalization and expression of Hsp27 in mouse oocytes from GV to MII cultured in vitro. (A) Immunofluorescence staining of Hsp27 and chromosomes in maturing oocytes. Oocytes culturing for 0 h (GV), 3 h (GVBD), 8 h (MI) and 14 h (MII) in vitro were stained with specific Hsp27 antibody (green) and propidium iodide (red). (B) Real time RT-PCR analysis of Hsp27 mRNA in maturing oocytes. Oocytes were cultured in vitro for 0, 3, 8 and 14 h. A progressive increase was observed with the maturation of oocytes. (C) Western blot analysis of Hsp27 expression in maturing mouse oocytes. Experiments were repeated at least three times. Bars = 20 μm. *P < 0.05, **P < 0.01 vs. control.Back to article page