Figure 3
Effect of rh adiponectin on proliferation of bovine granulosa cells. [3H] thymidine incorporation was determined in bovine granulosa cells cultured for 24 h in enriched McCoy's 5A medium (without FBS) supplemented with or without rh adiponectin (10 μg/mL), ± IGF-I (10-8 M), or insulin (10-8 M). The data are expressed as mean ± SE and the measure unit of [3H] thymidine incorporation is counts per min (CPM). The results are representative of three independent cultures with each condition in triplicate. Bars with different superscripts are significantly different (P < 0.05).