Cyclic AMP generation in response to EP receptor agonists. Germinal vesicle (GV) stage oocytes obtained from monkeys (6-10 oocytes/group) and mice (15-20 oocytes/group) were pooled and maintained in culture for 4 hours in the presence of vehicle, the EP2 selective agonist butaprost, the EP4 selective agonist PGE1-OH, or PGE2. Media + oocytes were stored at -20°C until assayed for cAMP by EIA. Data are expressed as fmol cAMP/oocyte. Within each species, data were analyzed by ANOVA and Duncan's Multiple Range Test. Groups with different letters are different, p < 0.05. Data are expressed as mean + SEM, n = 3-10 groups of oocytes/treatment.