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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Differential genome-wide gene expression profiling of bovine largest and second-largest follicles: identification of genes associated with growth of dominant follicles

Figure 4

Localization of GADD45A, IGFBP5 and TSP2 mRNA in healthy and atretic follicles. These genes were expressed more in F2 than in F1 in QPCR analysis. (A, C, E, G, I and K) DIG-labeled anti-sense cRNA probes were used. (B, D, F, H, J and L) DIG-labeled sense cRNA probes were used. Seven-micrometer sections of bovine follicles were hybridized with each probe. GADD45A (A, B, C and D) and TSP2 (I, J, K and L) mRNA were found in both granulosa cells (GC) and theca layer (TL) of atretic follicle, whereas it was localized in only GC of healthy follicle. IGFBP5 mRNA (E, F, G and H) was localized in GC and TL of atretic follicle but not found in healthy follicle. Scale bar = 20 μm.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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