Figure 3
From: Autocrine regulation of human sperm motility by tachykinins
Localization of neprilysins in human sperm. (A) Immunofluorescence and corresponding differential interference contrast images of sperm cells stained with primary antibodies against neprilysin (NEP) and neprilysin-2 (NEP2). Experiments were performed at least six times with similar results. Scale bar, 10 μM. (B) Western Blot analysis of NEP and NEP2 in human spermatozoa (SPZ) and seminal plasma (SP) homogenates. For NEP2, SP and SPZ1 represent results obtained with the NEP2 antibody sc-104450 and SPZ2 represents results obtained with the NEP2 antibody HPA 007876 (see text for further details). Results are representative of at least five separate protein preparations, each from eight different donors.