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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: CABYR isoforms expressed in late steps of spermiogenesis bind with AKAPs and ropporin in mouse sperm fibrous sheath

Figure 2

2-D diagonal gel Western blot of mouse sperm extract. A: Immunoblots probed with guinea pig anti-CABYR-A polyclonal antibodies. The presence of spots below the diagonal showed that the band running at ~70 kDa under non-reducing condition was broken down to ~50 kDa under reducing conditions; ~110-130 kDa bands resolved into 80 kDa and ~50 kDa forms; and a ~200 kDa band decomposed into 80 kDa and 50 kDa components. B: Probing by guinea pig anti-CABYR-B polyclonal antibodies showed that most of the high MW bands under non-reducing condition were broken down to 50 kDa and 40 kDa under reducing conditions. It may be concluded that the 50 kDa band contains both CABYR-A and CABYR-B. Principal CABYR monomers migrate at 80, 50 and 40 kDa.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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