Figure 6

Localization (A) and regulation (B) of mPRβ and γ in the human fallopian tube during the normal reproductive cycle. The expression of mPRβ (A, a), β-tubulin (A, b) and mPRγ (A, d-e) are visualized by immunohistochemistry using specific antibodies. Subpanels c and f are control sections where the primary antibody was omitted. Another membrane bound protein, the FSH receptor (FSH) and the nuclear progesterone receptors were also analyzed to confirm that the sample preparation indeed contained membrane proteins. Sampled were obtained from patients in the follicular (group I), periovulatory (group II) and the luteal (group III) phase of the menstrual cycle. Protein levels of the mPRs are presented in the bar graph as arbitrary densiometric values normalized to whole protein in Coomassie blue staining. Values are presented as mean ± SEM of four independent observations. For mPRγ, assumptions for the general linear model was not met despite transformation of data (unequal variances), thus three independent t-tests (allowing unequal variances) were applied to compare stages I, II and III followed by a Bonferroni correction for multiple comparisons. The statistical significance is shown as * P < 0.05.