Figure 3
Steroid-dependent regulation of mPRβ mRNA and mPRγ mRNA (A and B) and protein (C) in the fallopian tube of immature female mice. Mouse ovary was included as a positive control in (A). Twenty-one-day-old female mice received an injection of E2, P4, the estrogen antagonist ICI 182,780, the progesterone antagonist CDB2914 or oil and sampled two or six hours later. The levels of mRNA are normalized to the endogenous control (GAPDH) and expressed in relative numbers to the control group as means ± SEM of five independent observations (B) whereas protein levels are presented as mean ± SEM of three independent pools, each consisting of tissue from ten mice (C). Groups differing significantly from the control group are indicated by asterisks (** for P < 0.01 and * for P < 0.05)