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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Prolactin signaling through the short isoform of the mouse prolactin receptor regulates DNA binding of specific transcription factors, often with opposite effects in different reproductive issues

Figure 5

DNA binding activity of RUNX1. Day 9 pseudopregnant PRLR-/-RS mice were injected with ergocryptine (200 μg, sc) for 6 hr followed by a single ip injection of either PRL (60 ug) or vehicle (control). Decidua were obtained after 0, 30 and 120 min of PRL treatment. Nuclear extract from decidua was subjected to EMSA using oligonucleotide specific probe to RUNX1. Lane 1, 3 and 5 are nuclear extracts isolated from decidua of 0, 30 and 120 min PRL treated animals, respectively. Lane 2, 4 and 5 are nuclear extracts treated with competitor, a 50-fold molar excess of unlabeled probe.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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