Table 1 Nucleotide sequence of the specific primers used in PCR amplifications.
From: Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
Gene | Primers | Sense | Product Size |
|---|---|---|---|
Tac1 | 5'-GGCCAAGGAGAGCAAAGA-3' | F | 88 bp |
| Â | 5'-CGAGGATTTTCATGTTCGATT-3' | R | Â |
Tac2 | 5'-TCTGGAAGGATTGCTGAAAGTG-3' | F | 302 bp |
| Â | 5'-GTAGGGAAGGGAGCCAACAG-3' | R | Â |
Tac4 | 5'-GTAGCTTCCTCAGCCATGCAG-3 | F | 186 bp |
| Â | 5'-CCGCCCCCAAATACAATACA-3' | R | Â |
Tacr1 | 5'-GCCAGAACATCCCAACAGG-3' | F | 223 bp |
| Â | 5'-GGCGAAGGTACACACAACCA-3' | R | Â |
Tacr2 | 5'-TGGTACTGGTGGTGGTGACATT-3' | F | 251 bp |
| Â | 5'-CCTGTCTTCCTCGGTTGGTG-3' | R | Â |
Tacr3 | 5'-CCAACTACTGCCGCTTCCA-3' | F | 272 bp |
| Â | 5'-GAAATGTTGCTTGGGACCTTCT-3' | R | Â |
Actb | 5'-TCCCTGGAGAAGAGCTACGA-3' | F | 362 bp |
| Â | 5'-ATCTGCTGGAAGGTGGACAG-3' | R | Â |
Gapdh | 5'-CAATGCCTCCTGCACCAC-3' | F | 350 bp |
| Â | 5'-CCTGCTTCACCACCTTCTTG-3' | R | Â |
Ppp1cb | 5'-AACCATGAGTGTGCTAGCATCA-3' | F | 472 bp |
| Â | 5'-CACCAGCATTGTCAAACTCGCC-3' | R | Â |
Polr2a | 5'-CGTTTCCATCCTAAGCCCAGT-3' | F | 260 bp |
| Â | 5'-ATCTCTGCCCGTGTTTCCAG-3' | R | Â |