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Table 1 Nucleotide sequence of the specific primers used in PCR amplifications.

From: Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus

Gene Primers Sense Product Size
Tac1 5'-GGCCAAGGAGAGCAAAGA-3' F 88 bp
  5'-CGAGGATTTTCATGTTCGATT-3' R  
Tac2 5'-TCTGGAAGGATTGCTGAAAGTG-3' F 302 bp
  5'-GTAGGGAAGGGAGCCAACAG-3' R  
Tac4 5'-GTAGCTTCCTCAGCCATGCAG-3 F 186 bp
  5'-CCGCCCCCAAATACAATACA-3' R  
Tacr1 5'-GCCAGAACATCCCAACAGG-3' F 223 bp
  5'-GGCGAAGGTACACACAACCA-3' R  
Tacr2 5'-TGGTACTGGTGGTGGTGACATT-3' F 251 bp
  5'-CCTGTCTTCCTCGGTTGGTG-3' R  
Tacr3 5'-CCAACTACTGCCGCTTCCA-3' F 272 bp
  5'-GAAATGTTGCTTGGGACCTTCT-3' R  
Actb 5'-TCCCTGGAGAAGAGCTACGA-3' F 362 bp
  5'-ATCTGCTGGAAGGTGGACAG-3' R  
Gapdh 5'-CAATGCCTCCTGCACCAC-3' F 350 bp
  5'-CCTGCTTCACCACCTTCTTG-3' R  
Ppp1cb 5'-AACCATGAGTGTGCTAGCATCA-3' F 472 bp
  5'-CACCAGCATTGTCAAACTCGCC-3' R  
Polr2a 5'-CGTTTCCATCCTAAGCCCAGT-3' F 260 bp
  5'-ATCTCTGCCCGTGTTTCCAG-3' R  
  1. Shown are the structures of forward (F) and reverse (R) primers of indicated target genes and the size expected for each PCR-amplified product. Primers for Actb (β-actin), Gapdh, Ppp1cb and Polr2a, used as housekeeping genes, are also shown.