Figure 3
From: Characterization of a novel telomerase-immortalized human endometrial stromal cell line, St-T1b
Induction of KAI1 and p53 protein expression in decidualizing St-T1b cells. A) Total protein was harvested from St-T1b cells after 1, 3, or 7 d of treatment with 8-Br-cAMP and from untreated cells. Western blot analysis was performed for KAI1 and p53, and for GAPDH as a loading control. B) RNA was harvested from the same cultures as described in panel A and analyzed for KAI1, p53 and GAPDH transcript levels by RT-PCR.