Table 1 Optimal concentrations of Glutathione peroxidase reaction medium.

Potassium phosphate, containing 2,5 mM Na₂EDTA, 2,5 mM sodium azide, pH 7.0

0,5 M

Glutathione reductase (from baker yeast, ammonium sulfate suspension, 100–300 U/mg protein) in the same phosphate buffer;

0,18 U/ml: at 25°C

GSH (>98%) in distilled water

100 mM

NADPH (~95%) in 0,1% NaHCO₃ solution

10 mM

Cumene hydroperoxide (>80%) in distilled water

60 mM