Seminiferous tubule transfection in vitro to define post-meiotic gene regulation

Table 2 Summary of the amplified promoter sequences, the oligonucleotide PCR primers used, and their respective annealing temperatures.

Gene promoter	Ref	Sequence	PCR primers	PCR product	Annealing temperature
SP10	[9, 21]	-403 to +28	ratSP10_Ase_f ratSP10_r	437 bp	55°C
Proacrosin	[7, 26]	-263 to +48	ratProacro_Ase_f ratProacro_r	818 bp	62°C
Protamine 1	[8]	-556 to +30	ratPrm1_Ase_f ratPrm1_r	593 bp	55°C
Endozepine-like peptide (ELP)	[5, 19]	-652 to +51	ratELP290_Ase_f ratELP980_r	708 bp	55°C
		-957 to +617	ratELP10_Ase_f rat ELP1500_r	1571 bp	62°C
		-957 to +51	ratELP10_Ase_f ratELP980_r	1005 bp	60°C
		-652 to +617	ratELP290_Ase_f ratELP1500_r	1274 bp	60°C
		-652 to +51	ratELP290Bgl_f ratELP980_r	708 bp	60°C
		-652 to +617	ratELP290_Bgl_f ratELP1500_r	1274 bp	62°C
		-380 to +617	ratELP-GCNF_Bgl_f ratELP1500_r	1003 bp	60°C

Oligonucleotide primers (see Table 1) were used in the combinations indicated with rat genomic DNA as template to generate PCR products of the correct size. The sequence parameters are given relative to the transcription start site (+1) determined from the corresponding homologous mouse sequences, given in the references. PCR conditions are given in the text.

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