Integrin-linked kinase can facilitate syncytialization and hormonal differentiation of the human trophoblast-derived BeWo cell line

Table 3 The incidence of β-human chorionic gonadotropin (β-hCG) expression. The incidence of β-hCG expression was determined by the presence of β-hCG immunolocalization in groups of EGFP-fusion protein expressing cells. Data are from three independent experiments.

Experiment	BeWo + pEGFP-C3	BeWo + pEGFP-C3- dn-ILK	BeWo + pEGFP-C3- wt-ILK	BeWo + pEGFP-C3- ca-ILK
1	16/40	13/68	27/47	46/57
2	23/57	8/51	30/47	49/61
3	20/43	15/55	22/36	38/55
Avg. Incidence (% of 1.00 +/- SEM)	0.43 +/- 0.02	^a0.22 +/- 0.03	^b0.62 +/- 0.02	^{b, c}0.77 +/- 0.04

a: Cytoplasmic β-hCG expression markedly decreased in cells expressing dominant negative (dn)-ILK relative to pEGFP-C3 (empty vector) cells (p < 0.05)
b: Expression was significantly increased in cells expressing wildtype (wt)- and constitutively active (ca)-ILK compared to cells expressing empty vector or dn-ILK (p < 0.05)
c: Cytoplasmic β-hCG expression was significantly increased in cells expressing ca-ILK compared to cells expressing wt-ILK (p < 0.05)

ISSN: 1477-7827