Figure 1

Effect of LH on expression of mRNA for 11β-HSD2. Leydig cells were treated with increasing concentrations of LH for 24 h (A). The other cells were treated with LH for 2–24 h (B). At end of treatment, total cellular RNA was isolated, and the steady-state level of 11β-HSD2 mRNA was assessed by relative quantitative RT-PCR, as described in Methods. The expressions of mRNA for 11β-HSD2 in Leydig cells treated with 2.5, 5, 10, or 20 ng/ml LH for 24 h, or 20 ng/mL LH for 8, 12 or 24 h are significantly higher compared to that in intact Leydig cells. Each data point is expressed as percentage of control. Asterisks denote significant differences, compared with Control at P < 0.05.