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Table 6 IFN-γ ELISPOT assay in IncB or IncC stimulated CD4+ T cell cultures

From: Host immune responses to chlamydial inclusion membrane proteins B and C in Chlamydia trachomatis infected women with or without fertility disorders

 

GI

GII

GIII

 

IncB (μg/ml)

IncC (μg/ml)

IncB (μg/ml)

IncC (μg/ml)

IncB (μg/ml)

IncC (μg/ml)

 

0.5

1

0.5

1

0.5

1

0.5

1

0.5

1

0.5

1

Cervical cells

111

(48–214

126

(67–263)

91

(29–167)

142

(43–211)

132 a

(47–312)

157 b

(56–343)

112 c

(38–112)

178 d

(77–329)

43

(12–59)

49

(12–88)

38

(17–63)

59

(26–76)

PBMCs

121

(38–200)

134

(45–212)

103

(48–176)

172

(44–176)

212 a

(56–273)

226 b

(76–301)

243 c

(91–322)

251 d

(100–316)

56

(38–97)

67

(43–115)

63

(59–108)

81

(60–149)

  1. Note. Median number of IFN-γ SFCs per 7.5 × 104 CD4+ T cells at 18 h post stimulation with IncB or IncC (0.5 μg/ml and 1 μg/ml) counted using ELISPOT. Figures in parenthesis depict range of SFCs for each group.
  2. a, b, c, d P < 0.05 Number of IFN-γ in GII compared to respective levels in group III by Mann Whitney U test where, Group I (GI) comprised of healthy fertile women with no CT infection, Group II (GII) comprised of CT-positive fertile women.
  3. Group III (GIII) comprised of CT-positive women with fertility disorders.
  4. IncB- CT Inclusion membrane protein B; IncC- CT Inclusion membrane protein C; CT- Chlamydia trachomatis; EB-Elementary bodies; IL-Interleukin; IFN-γ-Interferon gamma; SFCs-Spot forming cells.