Figure 6
Estimation of IFN-γ mRNA relative expression in 7.5 × 104 CD4+ T cells isolated from cervical cells and PBMCs after in vitro stimulation with IncB or IncC. Real-time RT-PCR analysis of IFN-γ mRNA levels was done at 12 hours post stimulation in CD4+ T cells isolated cervical cells and PBMCs isolated from GI, GII and GIII where, Group I (GI) comprised of healthy fertile women with no CT infection, Group II (GII) comprised of CT-positive fertile women, Group III (GIII) comprised of CT-positive women with fertility disorders. * P < 0.05 mRNA expression of IFN-γ in GII compared to respective levels in group III by Mann Whitney U test. All cytokines were normalised against corresponding levels of β-actin endogenous gene. The graph show results as fold change represented by bars. Bars represent mean ± s.e.m. for all experiments.