Figure 3
From: Effects and mechanisms of action of sildenafil citrate in human chorionic arteries
Immunohistochemical demonstration of the localization of PDE-5 protein in chorionic plate arterial rings. Haematoxylin and eosin staining of a paraffin embedded 15 μm section from a chorionic plate arterial ring demonstrates the structure of the ring (Panel A). Subsequent images, enlarged from the section of the wall of this ring indicated by the rectangle, demonstrate nuclear staining with DAPI (Panel B), staining with PDE-5 primary and phycoerythrin labeled secondary (Sigma) antibodies (Panel C), and the superimposition of panels B and C to demonstrate the cytoplasmic location of PDE-5 (Panel D).