Table 1 CD46 primers used for RT-PCR, 5'-RACE and 3'-RACE reactions where amplicons were subsequently sequenced.

RT-PCR

CCTTCTGTTTCTGCTGTCT

CD46 exon 1, sense

Mm, As

AATCATACATGGGTCCCTA

CD46 exon 2, sense

Mm, As

TAGGGACCCATGTATGATT

CD46 exon 2, anti-sense

Mm, As

ACTACATAGATGGCAG

CD46 exon 3, sense

Mm, As

CTGCCATCTATGTAGT

CD46 exon 3, anti-sense

Mm, As

ATTGTTTACCACCTCCA

CD46 exon 5, sense

Mm, As

TGGAACACACACCTTTAC

CD46 exon 5, sense

As

CGATCAAAGCAACAATC

CD46 exon 9, anti-sense

As

TTCATCTTGCTGCAGATAC

CD46 exon 11, anti-sense

Mm, As

3'-RACE

TGGAACACACACCTTTAC

Gene-specific primer 1 (GSP), CD46 exon 5

As

AAATGTATCTGCAGCAAG

GSP2, CD46 exon 11

Mm, As

GACTCGAGTCGACATCGA

Anchor primer (AP)

-

GACTCGAGTCGACATCGA(T)₁₇G

AP-polyT-G

-

5'-RACE

AATTTAGCTCGAGCACC

GSP3, CD46 exon 3

Mm

CTGCCATCTATGTAGT

GSP4, CD46 exon 3

Mm, As

TAGGGACCCATGTATGATT

GSP5, CD46 exon 2

Mm, As

GACTCGAGTCGACATCGA

AP

-

GACTCGAGTCGACATCGA(G)₁₇

AP-polyG

-