hCG stimulation of Kitl expression in mouse cumulus and mural granulosa cells. hCG regulation of Kitl and c-kit expression in oocytes (OC), cumulus cells (CC) and mural granulosa cells (GC) at different times before and after hCG treatment of immature mice. Transcript levels of Kitl and c-kit in isolated ovarian cells were quantified by real-time RT-PCR. (Mean ± SEM, n = 4). Data were normalized based on histone H2a transcript levels. *, P < 0.05 vs.0 h of hCG treatment.