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Table 2 Development of reconstructed cat oocytes activated by electroporation or thimerosal/DTT.

From: Analysis of cat oocyte activation methods for the generation of feline disease models by nuclear transfer

Donor Cell type

Activation treatment

Embryos produced

Embryos cleaved and transferred (%)

Recipients

Pregnant recipients

Implantation sites (%)

No. of fetuses (%)

MPS

Electroporation

157

114 (72.6)

9

2*

4 (3.5)

3 (2.6)

AMD

Electroporation

73

62 (84.9)

6

2

4 (6.5)

2 (3.2)

AMD

Thimerosal/DTT

57

44 (77.2)

4

0

0 (0)

0 (0)

  1. Stimulated oocytes in both cases were subsequently incubated in cycloheximide and cytochalasin B for 4 h.
  2. *A third female had a single implantation site but with no observable fetus
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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