Table 1 Characteristics of [Ca2+ ]i changes (mean ± SEM) induced by various artificial stimuli
Treatment | No. of oocytes (No. of responses) | Time till response (sec) | Peak [Ca2+ ]i (nM) | Duration (sec) | Oscillation | Interval between peaks (sec) |
|---|---|---|---|---|---|---|
Electroporation | 15 (15) | 3 ± 0.4 | 485 ± 28 | 73 ± 15 | No | -- |
7% ethanol | 5 (12) | 110 ± 31 | 394 ± 87 | 150 ± 41 | No | -- |
5 μM ionomycin | 5 (5) | 18 ± 2 | 277 ± 27 | >800 | No | -- |
50 μM ionomycin | 5 (5) | 6 ± 1 | 336 ± 12 | >800 | No | -- |
200 μM thimerosal | 15 (18) | 365 ± 51 | 496 ± 75 | 85 ± 8 | Yes | 351 ± 30 |
20 mM SrCl2 | 15 (15) | 85 ± 10 | 431 ± 52 | 335 ± 28 | No | -- |
Na+-free medium | 15 (28) | 135 ± 24 | 639 ± 79 | 1470 ± 242 | No | -- |