Figure 4

Arrest of cell cycle in G2/M phase induced by progesterone and Alp. Human endometrial cells were treated with progesterone alone or in combination with Alp. Cell cycles were analyzed by flow cytometry. A: Cell cycle analyzed with propidium iodide (PI) staining followed by flow cytometry. B: Comparison of the proportions of cells in G2/M phase. The proportion of cells in G2/M phase was significantly increased after hECs were treated with progesterone or progesterone and Alp (*P < 0.05, **P < 0.01, compared with the control cells). There was also significant difference between treatment with progesterone alone and progesterone plus Alp (#P < 0.05).