Human male gamete endocrinology: 1alpha, 25-dihydroxyvitamin D3 (1,25(OH)2D3) regulates different aspects of human sperm biology and metabolism

Table 3 Effects of 1,25(OH)2D3 on sperm metabolism

	Triglycerides mg/10⁶ sperms	Lipase activity (U/L)mmol/min/ mg protein	Acyl CoA dehydrogenase activity nmol/min/mg protein	G6PDH mmol/min/10⁶ sperms
NC	0,08 ± 0.0013	0,5 ± 0.02	0,004 ± 0.0001	0,005 ± 0.0001
1,25(OH)2D3 0.01 nM	0,05 ± 0.001*	1,3 ± 0.04*	0,0039 ± 0.0001	0,0048 ± 0.0002
1,25(OH)2D3 0.1 nM	0,025 ± 0.0029**	1,8 ± 0.03**	0,0041 ± 0.0002	0,0047 ± 0.0002
1,25(OH)2D3 1 nM	0,015 ± 0.0032**	0,8 ± 0.032*	0,0040 ± 0.0003	0,0051 ± 0.00018
1,25(OH)2D3 10 nM	0,07 ± 0.002	0,6 ± 0.031	0,0038 ± 0.0001	0,0046 ± 0.0001
Ab VDR + 0.1 nM 1,25(OH)2D3	0,077 ± 0.0019	0,4 ± 0.041	0,0038 ± 0.0002	0,0049 ± 0.0002
CAP	0.06 ± 0.002	2,2 ± 0,045**	0,01 ± 0,0005**	0.01 ± 0,0003*

Data are presented as mean ± S.D. of seven independent experiments performed in duplicate.
*P < 0.05 versus control; ** P < 0.01 versus control (NC). CAP = capacitated sperms.

ISSN: 1477-7827