Figure 5

Immunoprecipitation of c-MET with PB1. Representative Western blots from whole cell extract of HEC-1A, RL95-2 and HEC-1A-2 cells. Endometrial cells were lysed, and protein in equal amounts (500 μg) was subjected to immunoprecipitation with PB1 antibodies (IP: PB1), followed by Western blotting with c-MET antibody (WB: c-MET). c-MET was observed mainly in immunoprecipitates from RL95-2 and HEC-1A-2. Lower lane represents negative control of PB1 immunoprecipitation, and Western blotting with GAPDH antibody (WB: GAPDH).