Figure 1

PR expression in endometrial cell lines. (A) Transcript expression level of PRB in HEC-1A versus RL95-2 cells. Representative agarose gel of RT-PCR for PRB and GAPDH in HEC-1A and RL95-2 cells. Product size: PRB 196 bp, GAPDH 230 bp. (B) Bar graph describing the mean ± SEM of the ratio between the expression level of PRB/GAPDH, black bars represent HEC-1A, white bars represent RL95-2, * P < 0.05, ND not detected. (C) Representative Western blots from nuclear and cytosol extracts of PRB (116 kD), PRA (82 kD) and PRC (60 kD) expression patterns. (D) Bar graph describing mean ± SEM of the percentage of PRB/PRA ratio in HEC-1A from RL95-2, black bars represent HEC-1A, white bars represent RL95-2, * P < 0.05.