The effect of forskolin on Ets-2 and p53 expression in 6-8 w 1st trimester trophoblasts. A, Representative photos taking with a confocal microscope of 6-8 w 1st trimester trophoblast, magnification × 60. 6-8 w 1st trimester trophoblast, cultured with or without Forskolin (10 μM), were fixed and stained with a fluorescent antibody against Ets-2, p53 or MMP-2. B, Density of p53, Ets-2 and MMP-2 staining was quantified with Image software and presented by Bar graph, left-Ets-2, middle-p53, right-MMP-2. C, Binding of nuclear proteins extracted from 6-8 w 1st trimester trophoblast cells treated with or without Forskolin (10 μM) to dig-labeled oligonucleotides Ets and p53 was assessed with EMSA. Results represent 2 different experiments performed in triplicates.