Figure 1

Effect of forskolin on MMP-2 expression in JAR and 6-8 w trophoblast. Top panel, total RNA of MMP-2 isolated form JAR (A+B) or 6-8 w trophoblast (C) cultured with Forskolin (10 μM) for the indicated times was assessed by Northern blot (A) and semiquantitative reverse transcription-PCR (B+C). Bottom panel, band intensities were quantified with Densitometer system endowed with TINA software and the data presented as the ratio of MMP-2 to GAPDH (percent of control). The value represents the mean ± SEM from six different experiments performed in duplicates.