Figure 7

Localization of GDF-8 transcripts by in situ hybridization (A and B) and peptide by immunohistochemistry (C and D) in the hamster 72 h p.c . uterus. (A) Low magnification of uterine cross section to show that GDF-8 mRNA was confined to endometrial epithelium. (B) Insert magnified to show intense signals in epithelial cells. (C) Absence of signals in negative control of ISH with the sense probe. (D and E) Immunostaining localized GDF-8 peptide to the endometrial stroma (s), but not the myometrium (m) and epithelium (e) (Number of animals =3). Bar = 100 μm).