Table 1 Primer-specific conditions used for quantitative PCR analysis
From: Expression of nodal signalling components in cycling human endometrium and in endometrial cancer
Gene | Primer sequence (5'-3') | size (bp) | Mg2+(mM) | Anneal temp (°C) | Ex'sion time (s) | Read temp (°C)1 |
|---|---|---|---|---|---|---|
Cripto | F AAGATGGCCCGCTTCTCTTACAGT | 511 | 2 | 64 | 20 | 88 |
| Â | R AAAGTGGTAGTACGTGCAGACGGT | Â | Â | Â | Â | Â |
Nodal | F AGACATCATCCGCAGCCTACA | 330 | 3 | 59.2 | 20 | 88 |
| Â | R GTCCATCTGAAACCGCTCTAAG | Â | Â | Â | Â | Â |
Lefty A | F GGGAATTGGGATACCTGGAT | 206 | 3 | 62 | 25 | 80 |
| Â | R CTAAATATGCACGGGCAAGG | Â | Â | Â | Â | Â |
β-Actin | F CGAGCGCGGCTACAGCTT | 500 | 2 | 64 | 14 | 85 |
| Â | R TCATACTCCTGCTTGCTGATCC | Â | Â | Â | Â | Â |