Figure 1

Leptin inhibited 8-bromo cAMP-stimulated progesterone production in human granulosa cells. Cells were treated with increasing concentrations of leptin (10 ng/ml - 1000 ng/ml) in the presence or absence of 1 mM 8-bromo cAMP for 24 h. Media were collected and assayed for progesterone production by ELISA. Each data point in the figure represents the mean ± SEM of three independent experiments. * above the column indicates that those groups differ significantly from untreated control (P < 0.05); (ctrl = control).