Figure 2From: Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horseSubcellular localization of Ob-R (A) and Ob (B) proteins in an equine IVM/ICSI-derived embryo. The sample is representative of confocal investigations on 25 serial optical sections (step size = 0.45 μm) performed on all examined embryos. A 4-cell stage embryo is shown obtained after IVM culture in presence of 100 ng/ml leptin. In surface planes, in the lower (planes n° 1 to 5) and upper (planes n° 19 to 25) parts of the embryo, a labeling in clumps/clusters can be seen while in planes nearer the equatorial position (planes n° 6 to 18), an intense fluorescence is observed beneath the oolemma together with uniform distribution of Ob (A) and Ob-R (B) staining within the cytoplasm. Scale bar represent 60μm.Back to article page