Figure 3

Effects of TP, DEHP (10, 100 mg/kg BW/day) on histopathological changes in immature male rats exposed to EDs from PND 21 to PND 35. Testis tissues were fixed in Bouin's solution and immersed in neutral formalin solution. The fixed tissues were embedded in paraffin, sectioned at 5 μm and mounted on slides. These sections were stained with hematoxylin and eosin (i.e., HE) and histopathological changes were assessed under a light microscope. Dilatation of the tubular lumen (a: stained signals), degeneration of Leydig cells (b: stained signals), and disorder of germ cells (c: stained signals) were observed in the testes of immature male rats. Results are shown at a 100 × magnification (i.e., bar = 200 μm), a 200 × magnification (i.e., bar = 100 μm) and a 400 × magnification (i.e., bar = 50 μm).