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Table 4 EPS, MMP and progressive motility in raw sperm, in DGC and in DGC combined with MACS-ANMB prepared sperm

From: Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential

Criteria, subgroups

Raw sperm

DGC sperm

DGC+MACS sperm

Non Apoptotic Fraction

DGC+MACS sperm

Apoptotic Fraction

EPS (%A+), n = 36

20.0 ± 2.3 (5–68)

11.8 ± 1.4 (1–32)

-

-

EPS (%A+), n = 21

22.1 ± 3.4 (5–68)

13.1 ± 2.0 (1–32)

6.7 ± 1.2 (1–22)

67.8 ± 4.2 (25–94)

MMP disruption (%MMP-), n = 31

61.7 ± 3.2 (30–95)

44.2 ± 3.7 (16–86)

-

ND

MMP disruption (%MMP-), n = 15

57.9 ± 4.5 (30–91)

37.0 ± 5.0 (16–86)

24.7 ± 5.6 (6–82)

ND

Motility, n = 28 (%progressive)

48.4 ± 2.4 (17–71)

64.4 ± 3.8 (22–91)

67.9 ± 5.4 (0–97)

8.0 ± 2.6 (0–53)

  1. Values are means ± SEM (minimum-maximum values).
  2. All values are statistically significantly different when comparing DGC+MACS, DGC, raw samples (p < 0.05, paired t-test) except for the value of motility 67.9 compared to 64.4.