Figure 6

Effects of EGF on mRNA expressions for the EGF receptors, SCF, c-kit and neuregulin1. Total RNA was extracted from the testicular fragments containing spermatogonial stage cultured for 1 week in the absence (Control) or presence of either EGF (4000 ng/μl) or FSH (200 ng/μl), and analyzed by RT-PCR for expressions of ErbB1 (200 bp, 35 cycles), ErbB2 (150 bp, 35 cycles), ErbB4 (150 bp, 35 cycles), SCF (390 bp, 33 cycles), c-kit (200 bp, 40 cycles), CRD-NRG1 (300 bp, 45 cycles), and Ig-NRG1 (450 bp, 45 cycles). EF-1α (550 bp, 25 cycles) is the internal control.