Figure 6

(a) Representative phase contrast images (10×) of bovine endometrial stromal cells at 10 days post induction with osteogenic medium, where nodules formed and started to accumulate extracellular matrix mineral deposition as reflected by the presence of black nodules using von Kossa staining. (b) At 21 days post induction with osteogenic medium nodules increased of number, size, organization as well as mineral deposition. The level of mineral deposition (dark dots) starting from the centre of the nodules (green) in the stromal cells stained with von Kossa dye was evaluated using an Axiovert 200 microscope and the associated AxioVision LE Rel. 4.4 densitometry software (Zeiss, Jena, Germany).