Effect of insulin and hormones on IGFBP-1 and IGFBP-2 during simulated pregnancy. The effect of insulin (+/- Ins) and hormones (hCG and/or estradiol and progesterone) on IGFBP-1 (BP-1) and IGFBP-2 (BP-2) synthesis in culture media taken from endometrial explants of intact baboons treated long-term (day 24–25) with steroids to simulate pregnancy (n = 2). Media and tissue samples were analysed by western and northern blotting for IGFBP-1 (A & B) and IGFBP-2 (C & D), respectively, after 24 h (D-1) and 48 h (D-2) incubation. Values above each band in A and B represent their optical density. PF: Proteolytic fragment of IGFBP-1; E: Estradiol; P: Progesterone.