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Table 1 Oligonucleotide primers used in semi-quantitative RT-PCR analysis.

From: Upregulated mRNA expression of desaturase and elongase, two enzymes involved in highly unsaturated fatty acids biosynthesis pathways during follicle maturation in zebrafish

Gene

Amplicon

Sense 5'-3'

Anti-sense 5'-3'

fadsd6

336 nt

CATCACGCTAAACCCAACATC

GCTCTCCATAAACCTGACGAAA

elovl5

474 nt

TGGACACCTTCTTCTTCATCC

TCTTCTCGCTGGACATCACTC

h2afz

159 nt

AGTTTTGGAGTTGGCAGGAAAT

AGAGACTTGTGGATGTGTGGAAT