Figure 3From: Upregulated mRNA expression of desaturase and elongase, two enzymes involved in highly unsaturated fatty acids biosynthesis pathways during follicle maturation in zebrafishExpression of elovl5 and fadsd6 normalized to h2afz during the zebrafish follicles development from PV to ovulation stage. PV represents pre-vitellogenic stage, EV represents early and mid-vitellogenic stage, LV represents fully-grown but immature stage, M represents matured oocyte stage and O represents ovulated oocyte stage. – ve represents negative control, with sterile water as template for RT-PCR. Each value represents mean ± SEM of three PCR runs at the significant level P < 0.05 using Tukey's HSD. Mean values with different alphabets are significantly different. Representative of the electrophoretic images of RT-PCR is shown at the lower panel.Back to article page