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Table 1 Number of fresh or frozen spermatozoa bound to the zona pellucida per oocyte after incubation of spermatozoa with anti-GalTase antibodies, preimmune serum or without any additive.

From: The involvement of beta-1,4-galactosyltransferase and N-acetylglucosamine residues in fertilization has been lost in the horse

Groups   Anti-Galactosyltransferase antibodies Preimmune serum No additive
Fresh semen No. Oocytes 31 28 28
  Spermatozoa/oocyte (mean ± SEM, n = 3) 99 ± 10 97 ± 9 112 ± 9
Frozen semen No. Oocytes 37 37 36
  Spermatozoa/oocyte (mean ± SEM, n = 2) 10 ± 1a 19 ± 2b 7 ± 1a
  1. a, b: statistical difference between the columns in the same line (for fresh semen F = 0.72 and p = 0.49, for frozen semen F = 19.78 and p < 0.001)
  2. n: number of replicates