The involvement of beta-1,4-galactosyltransferase and N-acetylglucosamine residues in fertilization has been lost in the horse

Table 1 Number of fresh or frozen spermatozoa bound to the zona pellucida per oocyte after incubation of spermatozoa with anti-GalTase antibodies, preimmune serum or without any additive.

Groups		Anti-Galactosyltransferase antibodies	Preimmune serum	No additive
Fresh semen	No. Oocytes	31	28	28
	Spermatozoa/oocyte (mean ± SEM, n = 3)	99 ± 10	97 ± 9	112 ± 9
Frozen semen	No. Oocytes	37	37	36
	Spermatozoa/oocyte (mean ± SEM, n = 2)	10 ± 1^a	19 ± 2^b	7 ± 1^a

a, b: statistical difference between the columns in the same line (for fresh semen F = 0.72 and p = 0.49, for frozen semen F = 19.78 and p < 0.001)
n: number of replicates

ISSN: 1477-7827