Figure 3

Western blot analysis of StAR protein in vivo and in vitro. Western blot analysis of StAR protein on Day 16 and 22 of pregnancy, and Day 3 post-partum. A representative autoradiogram showing a single immunoreactive band (a) and corresponding statistical analysis (b). There was a significant change between Day 16 of pregnancy and Day 3 post-partum (P < 0.05; one-way ANOVA). Values without shared notations differ at P < 0.05 (LSD test). Western blot analysis of StAR protein on Day 16 of pregnancy following incubation without trophic support for 8 h or following treatment with recombinant rat TNFα (37.5 ng/ml). A representative autoradiogram showing a single immunoreactive band (c) and corresponding statistical analysis (d). Values are expressed in arbitrary density units and show mean ± SEM for all groups (n = 3). Asterisk denotes a significant difference between control and 8 h group (P < 0.05; t-test).