Impaired leukocyte influx in cervix of postterm women not responding to prostaglandin priming

Table 1 Oligonucleotide primers used for real-time PCR, template amount and their annealing temperatures.

Gene	Accession No. or Reference	Primer F = forward; R = reverse	Position	cDNA	Annealing step
MMP-2	BC002576	F: gtatttgatggcatcgctca	bp 1695-1714	40 ng	56°C/20s
		R: cattccctgcaaagaacaca	bp 1891-1871
			product bp 198
MMP-8	NM_002424	F: ctttcagggaaaccagcaac	bp 790-809	80 ng	55°C/20s
		R: gcttggtccagtaggttgga	bp 893-874
			product bp 104
MMP-9	BC006093	F: cgctaccacctcgaactttg	bp 1124-1143	40 ng	56°C/20s
		R: gccattcacgtcgtccttat	bp 1319-1300
			product bp 198
TIMP-1	NM_003254	F: tgacatccggttcgtctaca	bp 299-318	40 ng	56°C/20s
		R: tgcagttttccagcaatgag	bp 400-381
			product bp 102
TIMP-2	PMID 15231657 [50]	F: gagcctgaaccacaggtacca	bp 728-748	40 ng	58°C/15s
		R: tctgtgacccagtccatcca	bp 841-822
			product bp 114
PAF-R	NM_000952	F: cagagacacacggtcactgc	bp 44-63	40 ng	56°C/15s
		R: catgtgggaggagtcatgtg	bp 154-135
			product bp 111
S-1	NM_002997	F: ggagcaggacttcacctttg	bp 869-888	50 ng	59°C/30s
		R: cccagcacctctttcctgt	bp 1009-991
			product bp 141
ERα	NM_000125	F: cttgctcttggacaggaacc	bp 1581-1600	80 ng	56°C/20s
		R: tcctctccctgcagattcat	bp 1691-1672
			product bp 111
ERβ	NM_001437	F: tgcggaacctcaaaagagtc	bp 715-734	80 ng	56°C/20s
		R: catccctctttgaacctgga	bp 854-835
			product bp 140
GPR30	NM_001505	F: agactgtgaaatccgcaacc	bp 364-383	100 ng	57°C/15s
		R: aagtgagcctggcatttgt	bp 663-644
			product bp 300
Cyclophilin A	XM_004890	F: gtggtgtttggcaaagtgaa	bp 401-420	40 ng	56°C/20s
		R: tcgagttgtccacagtcagc	bp 516-497
			product bp 116

ISSN: 1477-7827