Figure 3
From: Calcitriol affects hCG gene transcription in cultured human syncytiotrophoblasts
Stimulatory effects of calcitriol on hCG secretion and gene expression in cultured syncytiotrophoblasts. A) Hormone secretion was determined by EIA after 6 hours incubation in the presence of increasing concentrations of calcitriol or its vehicle (-). B) Real time PCR analysis of hCGβ gene expression of calcitriol-treated cells. Results were normalized against GAPDH mRNA. Vehicle data were arbitrarily given a value of 1. Each bar represents the mean ± S.D. of triplicate cultures. *P < 0.05 vs. control.