Table 1 Primer sequences for qPCR.
Putative gene name and symbol | 5'-Forward primer-3' | 5'-Reverse primer-3' | Product size (bp) |
|---|---|---|---|
Genes identified by SSH | Â | Â | Â |
Factor in the germline α (figla) | TCTTGAAGAATGCGATCAACTATGC | CACTCACGTCTCCATCTCCTGC | 177 |
Zona pellucida protein X (zpx) | TTGTGACACCTTGTTCCGCC | TCGCAACTACACGCCCTTAGAC | 257 |
Zona pellucida protein C (zpc) | CAGGAAAGCATCCACAGTGAAGC | TTTGTTACGACATCCAATGACCC | 153 |
Egg envelope glycoprotein (eeg) | TGGTGTCATCTGTCTCTTGAGGC | TTTGAAGCAGTTCTACGGCTCG | 233 |
Somatic lipoprotein receptor (vldlro) | AACAGAGCAACAACCAGATGCC | TAGAACCAGTATCACGCCCTGC | 278 |
Cathepsin B, a (ctsba) | GAACACTGACTGGGGAGATAATGG | CTCTGATAGGGTGGAGTTTCCTTTC | 222 |
Cathepsin D (ctsd) | CGTCATCTTTGACTCCCGATCC | GCACAAGTTTCCATTTGCTTTTCTG | 175 |
Cathepsin Z (ctsz) | TCCCATCGTTCCCAAAACCTAC | TGTTCCCAAGGCAAAGCACG | 200 |
Serum lectin isoform 2 (lcal) | CAGGTTCTTATGGAGCACACGG | GTTCAAGCAAAGGTGGAAAAGGAC | 203 |
Rhamnose binding lectin STL3 (lrham) | ACCAACTCACCGACACCAACTG | CCCAATGGAACACACATCGTG | 290 |
Alveolin (alv) | ACAGAGAAATCACCTGAGCCCC | GGAGAAATAAAACCACTGCCTGC | 253 |
Ferritin H-3 (fth3) | ACTCTCCTTCCAGAACAAGCGAG | TTGGTGAGGTTGGTGATGTGG | 264 |
DnaJ subfamily A member 2 (dnaja2) | TGTCTGCCATCTAAGTGTTTCAACG | CGCTGTAAAAAGTGCGAGGGC | 278 |
Cyclin E (ccne) | GTTCAAAGGAATCCCAGCAGATG | TCACCGTAGCACAATAAACACCC | 215 |
Retinol dehydrogenase 1 (rhd1) | GTTCAGGCAGATTCACCACCC | CGATGACCCCGTTCAGGTTC | 210 |
Coatomer complex protein, epsilon (cope) | CAGATGTCTCCATTGTCTCAAGCAG | TGACCCAGCACTTAGGCAAAGC | 175 |
Anterior pharynx defective 1b (aph1b) | CTGCTGAAGAAGGCAAACGAAG | AGGAATCAAAGAACACCACACCC | 268 |
Candidate genes | Â | Â | Â |
Vitellogenin receptor (vldlr) | TCTGGAAGTGTGATGGAGAGAAGG | TCGCTGGGTGAACATTTAGCC | 337 |
Apolipoprotein E (apoe) | AGGGAGGACATCCAGACCAAGTTG | CGTTTCTTCAGTTTGCGGGTGTAG | 218 |
Lipoprotein lipase (lpl) | GTGCCTCAACTGCCGTAAGAAC | TGCCCCAAATGTCAGACCAG | 365 |
FSH receptor (fshr) | GACGCACATCAGAGTGTTTCCC | GTAGAACCCTCAGTCCAGTGTTGC | 242 |
Anti-Müllerian hormone (amh) | TCACTTTCACCAGTCACTCTCTGC | CACTTCTTGTTCCGTCACCAATC | 204 |
Gonadal soma-derived growth factor (gsdf) | CCAGAATCAAGAAGGAATACGCAG | TGGGAAAAGAGAGGAGAGCAGG | 317 |
Elongation factor-1α (ef1a) | CCCCTGGACACAGAGATTTCATC | AGAGTCACACCGTTGGCGTTAC | 409 |