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Table 1 Primers used for real-time RT-PCR

From: Food intake regulating-neuropeptides are expressed and regulated through pregnancy and following food restriction in rat placenta

mRNA

Primer Sequence (5'-3')

Product size (bp)

Accession Number

NPY

Forward

cgctctgcgacactacatca

157

NM_012614

 

Reverse

tttcatttcccatcaccaca

  

AgRP

Forward

tgtgggccctttattagacc

156

XM_574228

 

Reverse

ccatatggacccccaatgt

  

POMC

Forward

tccatagacgtgtggagctg

174

NM_139326

 

Reverse

gacgtacttccggggatttt

  

CART

Forward

gccctggacatctactctgc

201

NM_017110

 

Reverse

cactgcgcactgctctcc

  

HPRT

Forward

cagtcccagcgtcgtgatta

137

NM_012583

 

Reverse

agcaagtctttcagtcctgtc