Table 1 Primers used for real-time RT-PCR
mRNA | Primer Sequence (5'-3') | Product size (bp) | Accession Number | |
|---|---|---|---|---|
NPY | Forward | cgctctgcgacactacatca | 157 | NM_012614 |
Reverse | tttcatttcccatcaccaca | |||
AgRP | Forward | tgtgggccctttattagacc | 156 | XM_574228 |
Reverse | ccatatggacccccaatgt | |||
POMC | Forward | tccatagacgtgtggagctg | 174 | NM_139326 |
Reverse | gacgtacttccggggatttt | |||
CART | Forward | gccctggacatctactctgc | 201 | NM_017110 |
Reverse | cactgcgcactgctctcc | |||
HPRT | Forward | cagtcccagcgtcgtgatta | 137 | NM_012583 |
Reverse | agcaagtctttcagtcctgtc | |||