Figure 4
From: Expression of RHOGTPase regulators in human myometrium
a. Representative RT-PCR gel pictures of (i) ARHGEF1 (ii) ARHGEF11 (iii) ARHGEF12 (iv) ARHGAP5 (v) ARHGAP24 and (vi) MSN mRNA expression in human myometrial cells (lanes 1 and 2 of each gel and lanes 2–3 for MSN). The PCR negative control is indicated (C). The DNA marker 100 bp ladder (Promega, UK) is indicated (M). b. Representative western blots of (i) p-MSN and (ii) ARHGDIA protein expression in human myometrial cells (lanes 1–4 of each gel). The corresponding β-Actin (ACTB) western blot is presented underneath the appropriate blot. Molecular weights are indicated in kDa.