Table 2 PCR conditions. PCR reactions were performed with DynaEXT with standard protocols; exon 6 and 7 were amplified as one fragment.
From: Studies of a co-chaperone of the androgen receptor, FKBP52, as candidate for hypospadias
Exons 2, 3, 4, 5, 9, 10 | Exon 1 | Exons 6–7,8 | |
|---|---|---|---|
DNA | 25 ng | 25 ng | 25 ng |
10×Buffer w.MgCl2 | 2,5 μl | 2,5 μl | 2,5 μl |
10 mM dNTPs | 0,5 μl | 1 μl | 0,5 μl |
10 μM forward primer | 1 μl | 1 μl | 1 μl |
10 μM reverse primer | 1 μl | 1 μl | 1 μl |
DyNAzyme™ EXT | 0,5 U | 1 U | 0,75 U |
DMSO | 0 | 1 μl | 0 |
H2O | To 25 μl | To 25 μl | To 25 μl |