Figure 4
From: Oncostatin-M inhibits luteinizing hormone stimulated Leydig cell progenitor formation in vitro
Immunohistochemical labelling for the presence of LIF-R in the testis of 13-day-old rats (A-C). Faint brown staining for the presence of OSM type I receptor/LIF receptor is present in a cluster of fetal-type Leydig cells (A; asterisk). The staining is much stronger in stem Leydig cells/precursor cells identified by their spindle-shaped nucleus, located in close vicinity of the basal membrane of the seminiferous tubules (A, B; filled arrows) and in Leydig cell progenitors identified by their oval nucleus which are either found close to the seminiferous tubules (A, B) or blood vessels (C; arrowhead). Staining in spermatogonia was faint (B, C; open arrow). (D) Control in which the first antibody was replaced by rabbit serum. Scale bar represents 11 μm.